Optimization and Determination of Kinetic Parameters of the Synthesis of 5-Lauryl-hydroxymethylfurfural Catalyzed by Lipases

Hydroxymethylfurfural esters (HMF-esters) have great potential for additive development; this reason, the goal of work was to study optimization esterification conversion HFM and lauric acid using two lipases: Novozym 435® biocatalyst immobilized lipase from Thermomyces lanuginosus (TL). For conversion, a three-level three-factorial Box–Behnken experimental design used. The models achieved good fit (R2 over 90%) reactions catalyzed with TL lipase. best 78.4%, 30 mM HMF, 16 U biocatalytic activity, 50 °C. kinetic parameters without inhibition by substrate were determined Michaelis–Menten mechanism, whereby VMax both biocatalysts reached highest values at °C, enzyme–substrate affinities (low Km) temperatures °C 40 It can be concluded that has catalyze reaction since, under optimal conditions, an 80.6% (value predicted) could achieved.